WesternSure Eco ECL Chemiluminescent Substrate is a highly sensitive, enhanced substrate for detecting horseradish peroxidase (HRP) on immunoblots. It is designed for high sensitivity and long signal duration, making it ideal for use with imaging systems and film. This substrate provides stable, high light intensity and allows the detection of low-abundant proteins down to the mid-femtogram detection level.
This is a single-use formulation that reduces reagent contamination and water consumption.
The substrate is optimized for use with the C-DiGit® Blot Scanner, Odyssey® XF Imager, Odyssey Fc Imager, and Odyssey M Imager (Model 3350).
Specifications
The WesternSure Eco ECL Substrate PN 926-86000 contains 20 Luminol Enhancer Tablet and 20 Stable Peroxide Tablet.
The WesternSure Eco ECL Substrate PN 926-86010 contains 2 Luminol Enhancer Tablet and 2 Stable Peroxide Tablet.
For Research Use Only. Not for use in diagnostic procedures.
Storage
Stable for 1 year at 4 °C.
Performance
Figure 1: PANC-1 whole cell lysate, starting at 10 μg/well (2-fold serial dilution), was probed with a mouse anti-Vimentin primary antibody followed by WesternSure HRP-conjugated goat anti-mouse IgG. The image was acquired using an Odyssey M Imager with the chemiluminescence module (3350) and a 30-second exposure.
