The 2D Chemicals Bundle includes key reagents for protein sample preparation and 2D electrophoresis. Together, urea, CHAPS, DTT, and iodoacetamide support protein solubilization, denaturation, reduction, and alkylation during preparation for high-resolution protein separation. Purchasing these products together provides a convenient way to prepare for your 2D electrophoresis workflow at a savings compared with purchasing each product separately.
What’s Included:
- Urea, analytical grade, 5 kg (24524.03)
- A chaotropic agent used to solubilize and denature proteins for isoelectric focusing and 2D electrophoresis.
- CHAPS (17038.02)
- A mild, zwitterionic detergent used to solubilize proteins and reduce protein-protein interactions. Its lack of net charge across a broad pH range makes it suitable for isoelectric focusing and 2D sample buffers.
- Dithiothreitol (DTT), analytical grade, 5 g (20710.03)
- A reducing agent used to break protein disulfide bonds during sample preparation.
- Iodoacetamide, research grade (26710.01)
- An alkylating agent used during protein sample preparation to modify reduced cysteine residues.
- An alkylating agent used during protein sample preparation to modify reduced cysteine residues.
